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KMID : 0378019680110050083
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New Medical Journal
1968 Volume.11 No. 5 p.83 ~ p.94
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The Effect of Shaking Stress on the Lesions of the Stomach of Albino Rats
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Abstract
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Singer(1913) first produced lesions in the rumen(forestomach) of rats by feeding with bread mixed with wood sawings. Hereafter, a number of workers produced lesions in the rumen by various. methods.
Sharpless(1937, 1940) also produced lesions in the rumen of the rats by feeding with low protein diet. He described that the early lesion was characterized by a greatly thickened squamous epithelial layer that the thickning was accompanied by papilloma formation at the surface and hypertrophy of the papillae at the base, that ulceration of the epithelium might occur and the ulcer was usually superficial and that metastases of the lesion had never been found.
On the other hand, Shay et al.(1945) produced ulcers of forestomach in rats by ligation of the pylorus. The rat was fasted for 48 to 72 hours, and then pylorus was ligated for 16 to 18 hours.
Later Robert and Nezamis(1964) produced the same ulcers of forestomach without ligation of pylorus but only by fasting of the rat for four days.
Levrat and Lambert(1959) produced ulceration in the glandular portion by shaking stress and intermittent photic stimulation. But Lee(1964), Moon(1964) and Park (1967) produced lesions of the forestomach by shaking stress but without photic stimulation. They fed the rats on the diet of biologically low protein diet. Kim and Lambooy(1967) produced ulceration in the glandular portion by shaking stress but they fed the rats on riboflavin deficient diet.
The purpose of this experiment is to investigate the effect of ACTH and cortisone on the production of lesions in the rumen of rats fed on rice diet. The lesions of the forestomach was investigated in detail microscopically and esophagus was also investigated microscopically.
Materials and Methods
Apparently healthy male young albino rats were fed on rice diet from 10 to 15 days earlier from the beginning of the experiment. Then the rats were divided into two groups.
To the experimental groups cortisone and ACTH was intraperitoneaily injected everyday and other soup was used as control given the same amount of normal saline solution. The rats were shaken 4 hours daily according to the method of Moon(1964).
The rats were shaken with frequency of 180 per minute on the first day, and thereafter the frequency increased 20 per minute everyday until the frequency reached to 260 per minute. The shaking stress continued for 7 days. Then the rats were sacrificed and the stomach was examined macroscopically
In the escphagus, 4 cases of 54 experiments revealed the esophageal ulcerations and were histologically confirmed. Generally the ulcers found in the esophagus appeared only in the epithelial layers, and were characterized by hemorrhage; and erosion. The epithelial layers around the esophageal ulcerations revealed slight proliferative and cornified changes. But the lamina propria around the ulcer and the submucosa just below the ulcer revealed little immflammatory changes as those of the forestomach ulcerations.
Summary
The pathological changes of the forestomach produced by shaking stress in albino rats fed on low protein diet and those rats pre-treated with cortisone or. ACTH prior to shaking stress were investigated macroscopically and histologically. The following results were obtained in this study.
1. The lesions of the forestomach produced by shaking stress were accompanied by edema,
hyperplasia, and cornification of the gastric mucosa.
2. The pathological changes of the forestomach observed in rats treated with cortisone or ACTH
prior to shaking stress were less severe than those in rats pre-treated with only shaking stress.
3. Ulceration was also observed in the esophagus where inflammatory changes were slighter than
those of the forestomach.
Summarizing the above results, the lesions of the forestomach in albino rats fed on low protein diet were produced by shaking stress. It is likely that cortisone or ACTH act more or less as inhibiting agents to gastric ulceration of albino rats.
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